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Figure 1 | Molecular Brain

Figure 1

From: Identification of a single amino acid in GluN1 that is critical for glycine-primed internalization of NMDA receptors

Figure 1

Glycine treatment primes depression of wild type recombinant NMDA receptors mediated currents. A, Representative traces show responses to the test applications recorded from cells expressing GluN1/GluN2A that were treated with ECS (upper) or Glycine (lower). Glycine (100 μM) was applied for 5 min, where indicated. For the expanded traces on the right, time indicates time after glycine treatment. B, Histogram showing average normalized peak NMDA currents evoked by NMDA/glycine test applications 20 min after conditioning with glycine (100 μM) to both GluN2A and GluN2B expressing HEK293 cells. C, NMDAR internalization (mean ± s.e.m.; percentage of total) measured by cell ELISA assay in HEK293 cells expressing wild type recombinant NMDAR. Cultures (n = 6) were pre-treated with ECS or ECS containing glycine (100 μM) plus APV (100 μM) followed by ECS or with NMDA (50 μM) plus glycine (1μM). ** indicates p < 0.01 compared with ECS control.

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Molecular Brain

ISSN: 1756-6606