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Figure 2 | Molecular Brain

Figure 2

From: Identification of a single amino acid in GluN1 that is critical for glycine-primed internalization of NMDA receptors

Figure 2

Glycine treatment primes internalization and recruitment of adaptin β2 to the wild type recombinant NMDA receptors. A, Fluorescence images of HEK 293 cells expressing GluN2A and GluN2B containing NMDARs labeled with CypHer5E-conjugated BTX and BTX-AF488 treated with NMDA (50 μM) plus glycine (1μM) with or without glycine pre-treatment. Scale bar = 10 μm. B, Left Representative Western blot of co-immunoprecipitation of NMDARs with adaptin β2 from cell lysate treated with glycine (100μM) plus APV (100μM) or APV (100μM) alone. Right Quantification is summarized in the histogram showing mean GluN1-adaptin β2 association after glycine treatment (n = 10). Data are presented as mean percent (± s.e.m.) of APV treated group. Data were normalized to the amount of adaptin β2 immunoprecipitated. ** indicates p < 0.01 *** indicates p < 0.001 compared to control.

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Molecular Brain

ISSN: 1756-6606