Figure 4

Glycine primed NMDAR internalization and depression of NMDAR mediated currents require dynamin. A, Co-expression of mutant dynamin K44A (Dyn_K44A) but not wild-type dynamin blocks glycine primed depletion of surface NMDA receptors (n = 4). * p <0.05, **p <0.01, paired t-test. B, Representative traces show responses to the test applications recorded from cells expressing GluN1/GluN2A that were treated with vehicle (DMSO, upper) or treatment (Dynasore, lower). Glycine (100 μM) was applied for 5 min where indicated. In the expanded traces on the right, time indicates time after glycine treatment. The graphs show normalized NMDA-evoked peak currents from recordings with intracellular administration of vehicle or dynamin inhibitor dynasore (80 μM) before and after glycine conditioning (indicated by the bar above each graph) for cells transfected with GluN1/GluN2A (n = 4 cells; left panel) or with GluN1/GluN2B (n = 6 cells; right panel).