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Figure 2 | Molecular Brain

Figure 2

From: Interactions between αCaMKII and calmodulin in living cells: conformational changes arising from CaM -dependent and -independent relationships

Figure 2

Detection of the interaction of αCaMKII with CaM and resultant conformational changes of αCaMKII by FRET. (A, B, C) Interaction of YFP-αCaMKII with CFP-CaM. (D, E, F) Conformational change of YFP-αCaMKII-CFP. (A, D) Images of typical YFP/CFP ratio before (−iono) and 10 min after the application of ionomycin (0.5 μM) (+iono). KN-93 (2 μM) or KN-92 (2 μM) was incubated for 30 min before the application of ionomycin. (B, E) Relative emission ratios before and after the application of ionomycin in the cytoplasm. 40–50 cells were analyzed in each group. (C, F) FRET efficiency using acceptor photo-bleaching in the cytoplasm. 20–30 cells were analyzed in each group.

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