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Figure 2 | Molecular Brain

Figure 2

From: Tristetraprolin expression and microRNA-mediated regulation during simian immunodeficiency virus infection of the central nervous system

Figure 2

miR-29a-mediated regulation through the TTP 3′ UTR. HEK-293 T cells (A) or monocyte-derived macrophages (B) were transfected with reporter plasmids (GFP-TTP-3′ UTR or GFP empty vector), red fluorescent protein (RFP) transfection control, and small RNA molecules as indicated (scrambled mimic control, miR-29a mimic, antagonist control, or miR-29a antagonist). 24 hours post-transfection, fluorescence of cells transfected with reporter and empty vector were determined by flow cytometry. A) Exogenous miR-29a caused a significant reduction (***, p < 0.0001 by t-test, p < 0.01 by ANOVA) in the normalized fluorescence ratio of HEK-293 T cells transfected with the GFP-TTP-3′ UTR construct vs. GFP vector alone. B) Because of sample-to-sample variability, the consistent decrease in normalized GFP-TTP 3′UTR fluorescence in the presence of exogenous miR-29a in macrophages approached but did not reach statistical significance as determined by ANOVA (p > 0.05). Error bars are standard error of the mean.

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