Figure 4

Novel interactions of miRNAs with TTP 3′ UTR elements. A) Alignment representing the previously-described miR-29a/TTP 3′ UTR interaction, along with alignments for additional miR-29 family members and a novel predicted interaction with miR-145. In each alignment, TTP (top) and miRNA (bottom) canonical base pairings are shown with ‘|’, while non-Watson-Crick “wobble” pairing of G and U is shown as ‘:’. B. Reduction in mean fluorescence intensity (MFI) of the TTP (ZFP36) 3′ UTR in the presence of the transfected synthetic miRNAs (HEK-293 T cells). Error bars are standard error of the mean.