The hypothalamus tissue was prepared for the ChIP assay using antibodies specific for c-Jun and c-Fos. PCR amplification using primers designed against AP-1-binding sites was performed. Equal amounts of the soluble crosslinked chromatins present in each PCR were confirmed by the product for input. Rabbit polyclonal IgG was used as a negative control. Input, 1% of sonicated cross-linked chromatins. Upper panel: the result of ChIP analyzing AP-1/DNA binding activity. Lower panel: relative densitometric values for ChIP assay. Contents of AP-1/DNA binding activity were indicated as the percentage of the control group. Bars are the means ± SEM. N = 8 each group. * p < 0.05 vs. control.