Upper panel: The effect of Y1R inhibitor (BIBP-3226) on 24-h AMPH-induced appetite suppression. AMPH-induced appetite suppression could be modulated by prior administration of BIBP-3226 (80 nmol, i.c.v.). Lower panel: The effect of BIBP-3226 on the changes of hypothalamic NPY, c-Fos, and c-Jun in 4 mg/kg AMPH-treated rats during the first day of drug treatment. AMPH-induced changes of NPY, c-Fos, and c-Jun could be modulated by prior administration of BIBP-3226. Contents of NPY, c-Fos, and c-Jun were indicated as the percentage of controls. * p < 0.05 vs. the control (aCSF-treated, i.c.v.) group. #p < 0.05 vs. the AMPH-treated groups. Bars are the means ± SEM. N = 8 per group.