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Figure 1 | Molecular Brain

Figure 1

From: Exosomes neutralize synaptic-plasticity-disrupting activity of Aβ assemblies in vivo

Figure 1

Characterization of ADDLs and exosomes used for biochemical and physiological experiments. (A) ADDLs were analyzed by Western blotting using 6E10. SM, size markers (kDa). (B) DLS particle distribution analysis of ADDLs (red line) (14.3 ± 1.1 nm) and Aβ1-42 freshly dissolved in 10 mM NaOH (black line) (6.4 ± 0.3 nm) was expressed as hydrodynamic radii (R H ). (C) A tapping AFM mode image of ADDLs (X-Y, 5 x 5 μm with an inset displaying a z-range in color from 0 to 15 nm). (D) By AFM, only small (3 - 6 nm) globular structures were detected. (E) Exosomes isolated from the conditioned medium of N2a cells had their density 1.13 g/ml to 1.19 g/ml, and contained the exosomal marker proteins Alix, Flotilin-1 and PrPC. Multiple (non-, mono- or di-) glycosylated PrPC proteins were detected between 20 ~ 35 kDa on SDS-PAGE. (F) By EM, exosomes appeared as closed vesicles of 30-120 nm in diameter (Scale bar: 100 nm), (G) a size range that agreed with that measured by DLS.

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