Figure 3
From: Exosomes neutralize synaptic-plasticity-disrupting activity of Aβ assemblies in vivo
ADDLs bound to exosomes . (A) Immunoblots of ADDLs after incubation either with PBS or exosomes (left top). Exosomes present in each lane were verified with Flotillin-1 (left bottom). Right, relative optical density (O.D.) of Aβ species. (B) Immunoblot results of in vitro binding assay of Aβ and exosomes (left top). Either exosomes or ADDLs alone were loaded as controls. Left bottom, exosomes were also verified with an exosomal marker Flotillin-1 (P, pellet; S, supernatant). Right, relative O.D. of pelleted Aβ. (C) Exosome-bound ADDLs remained accessible to digestion by trypsin (left top). Limited proteolysis with trypsin resulted in cleavage of exosomal surface protein PrPC, but not the intraluminal protein Alix (left, middle and bottom). Very low amount of ADDLs remained after trypsin treatment and the levels of which were similar irrespective of the presence or absence of exosomes (right, relative O.D. of Aβ). Error bars, ± SEM. Statistical significance was expressed as *, P < 0.05; **, P < 0.01.