Reduced mGlu1 receptor mRNA and protein levels in the cerebellum of symptomatic SCA1 mice. mGlu1 receptor mRNA and mGlu1α receptor protein levels in the cerebellum of presymptomatic and symptomatic SCA1 mice (and their age-matched wild-type littermates) are shown in (A) and (B), respectively. Values are means ± S.E.M. of 3–4 mice per group. *p < 0.05 (Student’s t test) vs. the corresponding wild-type mice; t values = 5.3 (mRNA values normalized to GAPDH); 6.5 (mRNA values normalized to calbindin); and 7.075 (densitometric analysis of immunoblots). Immunohistochemical analysis of mGlu1α receptors and calbindin in the cerebellum of SCA1 mice and wild-type littermates are shown in (C) and (D).