Appearance of mGlu5 receptors in Purkinje cells of symptomatic SCA1 mice. mGlu5 receptor mRNA and protein levels in the cerebellum of presymptomatic and symptomatic SCA1 mice (and their age-matched wild-type littermates) are shown in (A) and (B), respectively. Values are means ± S.E.M. of 4 mice per group. *p < 0.05 (Student’s t test) vs. the corresponding wild-type mice. t = 10 (densitometric analysis of immunoblots). Immunohistochemical analysis of mGlu5 receptors in the cerebellum of SCA1 mice and wild-type littermates are shown in (C) and (D). Confocal microscopy analysis of mGlu5 receptors in Purkinje cells of wild-type and symptomatic SCA1 mice is shown in (E). Note the somatic and perisomatic immunostaining which excludes the cell nucleus.