Figure 1

CGCs are more sensitive to TM-mediated neurotoxicity than CNs. A. Murine primary CGCs and CNs were exposed to various concentrations of TM for 3 d. Newly synthesized glycans were labeled for 20 h with [³H]-mannose and assayed, n = 3, error bars: standard deviation. Synthesis of glycans was decreased considerably more in CGCs than in CNs. Treatment with 1 ng/mL TM in CGCs resulted in a reduction in glycan synthesis (25%) comparable to that by treatment with 25 ng/mL of TM in CNs (indicated by dashed lines). B. CGCs and CNs were exposed to various concentrations of TM for 3 d. Cells were then subjected to a TUNEL assay to quantify cell death. TM treatments induced cell death in both CNs and CGCs. **: p < 0.01; ***: p < 0.001. When glycan synthesis was inhibited at a comparable level (25%), cell death in CGCs treated with 1 ng/mL TM was significantly more than that of CNs treated with 25 ng/mL TM. #: p < 0.01. C. Representative TUNEL staining of CNs and CGCs upon treatment with 25 and 1 ng/mL TM, respectively. Dying cells were stained in green and cell nuclei were stained in blue (with DAPI). TUNEL staining of cells without TM treatment (TM0) was used as a control. Please note that during cell culturing, the seeding densities for CNs and CGCs were different.