Figure 2

Downregulation of PMM2 results in more cell death in CGCs than in CNs. CGCs and CNs were infected with lentivirus containing PMM2 or Scrambled control (SC) shRNAs for 3 d. Cells were then subjected to Western blotting and densitometry to determine the protein level reduction of PMM2, n = 3, *: p < 0.05; **: p < 0.01; ***: p < 0.001 (A); and TUNEL staining to study cell death (B). Dying cells were stained in red and cell nuclei were stained in blue (with DAPI). C. After TUNEL staining, cell death percentages were quantified. Downregulation of PMM2 led to increased cell death in both CNs and CGCs, *: p < 0.05; **: p < 0.01; ***: p < 0.001. However, there was considerably more cell death in CGCs than in CNs. ##: p < 0.01. Please note that during cell culturing, the seeding densities for CNs and CGCs were different. D. CGCs and CNs were infected with lentivirus containing PMM2 shRNA1 or SC shRNA for 3 d, and then subjected to PMM enzymatic activity assay, n = 3, *: p < 0.05.