TFLLR-induced conductance is independent on activation of gap-junction hemichannels and Ca2+activated potassium channel. A. Averaged current–voltage relationships from TFLLR-induced current using Cs-Glutamate pipette solution in the presence of gap junction blocker (+CBX) or normal ACSF bath solution (No treatment). B. Bar graphs indicates the averaged current amplitude at holding potential of -100 mV (Current amplitude @ -100 mV) to test whether glutamate-mediated current in astrocytes is reduced by inhibiting the gap junction activity. p = 0.7 for Current amplitude @ -100 mV; unpaired t-test.