Macrophages displayed different degrees of polarization during HD course. A and B, Bar histograms showing variable percentage of CCR2+CX3CR1- (M1) and CCR2-CX3CR1+ (M2) macrophages in pre-manifested subjects (pre-HD), severe HD patients (HD) and healthy controls (CTRL). C and D, Bar histograms showing changes in the percentage of IL-12 and IL-10 positive macrophages in pre-manifested subjects (pre-HD), severe HD patients (HD) and healthy control subjects (CTRL). C, IL-12-producing macrophages were increased in preHD subjects coherently with M1 phenotype. D, IL-10-producing macrophages were increased in HD patients, consistently with changes of M2 phenotype. Data are shown as mean ± s.d * p < 0.05 ** p < 0.001 (ANOVA, Tukey post-hoc test).