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Figure 4 | Molecular Brain

Figure 4

From: Involvement of the N-methyl-d-aspartate receptor GluN2D subunit in phencyclidine-induced motor impairment, gene expression, and increased Fos immunoreactivity

Figure 4

Analyses of Fos induction in various brain regions in wildtype and GluN2D knockout mice. The photomicrographs illustrate the immunohistochemical labeling of Fos in coronal sections of the thalamus. The boxes are shown in panels a-i are at higher magnification. A schematic representation of the paraventricular thalamic nucleus (PV), thalamus (Th), and subthalamic nucleus (Sth) is shown in panel H. The number of Fos-positive cells per square millimeter was determined in the respective brain areas (E–G) and normalized to the number in saline-treated mice (I). Open bars, wildtype (WT) mice; closed bars, GluN2D KO mice. The results are expressed as the mean ± SEM ratio between the density of Fos-positive neurons in PCP-treated mice to the density of Fos-positive neurons in saline-injected mice (n = 4–6). One-way ANOVA of the PCP-induced increase in Fos-positive cells indicated a significant effect of genotype in the thalamus (Kruskal-Wallis nonparametric ANOVA followed by Scheffe’s test, p = 0.0209, unequal variance). *p < 0.05. Scale bars = 1 mm and 200 μm in panel D and d, respectively. The brain regions are defined as indicated by the images in the atlas [10].

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