Figure 5
Analyses of Fos induction in various brain regions in wildtype and GluN2D knockout mice. The photomicrographs illustrate the immunohistochemical labeling of Fos in coronal sections of the forebrain. The boxes are shown in panels a-i are at higher magnification. A schematic representation of the cingulate cortex (Cg), motor cortex (M), and striatum (Str) is shown in panel H. The number of Fos-immunoreactive cells per square millimeter in PCP-treated mice was determined in the respective brain regions (E–G) and normalized to the number in saline-treated mice (I). Open bars, wildtype (WT) mice; closed bars, GluN2D KO mice. The results are expressed as the mean ± SEM ratio between the density of Fos-positive neurons in PCP-treated mice to the density of Fos-positive neurons in saline-injected mice (n = 4–6). One-way ANOVA of the PCP-induced increase in Fos-positive cells indicated a significant effect of genotype in the striatum (F1,6 = 75.8, p = 0.0001). *p < 0.05, ***p < 0.001. Scale bars = 1 mm and 200 μm in panel D and d, respectively. The brain regions are defined as indicated by the images in the atlas [10].