Figure 6

Quantitative RT-PCR demonstrated the transcription of c- fos expression in the thalamus and striatum. The ratio of c-fos/actin transcripts is expressed as the mean ± SEM fold change from saline controls in WT mice. (A) Two hours after the injection of UBP141 (3 mM, 20 μl, i.c.), a significant increase in c-fos mRNA was observed in the thalamus and striatum in WT but not KO mice (*p < 0.05). (B) CIQ (20 mg/kg, i.p.) markedly increased c-fos mRNA expression in the thalamus and striatum in WT and KO mice (**p < 0.01). No genotype difference was found between WT and KO mice. (C) PCP (10 mg/kg, s.c.) significantly increased c-fos mRNA expression in the thalamus with or without dantrolene (**p < 0.01), which completely suppressed PCP-induced hyperlocomotion. In the thalamus, dantrolene (5 mg/kg, i.p.) itself induced the slight activation of c-fos expression in GluN2D KO mice (*p < 0.05). (D) PCP (10 mg/kg, s.c.) significantly increased c-fos mRNA expression in the striatum in WT mice with or without dantrolene, but PCP had no effect in the striatum in GluN2D KO mice.