Run-up of Piezo2 mechanically activated currents in HEK293 cells. A) Two sets of sample traces of MA currents in a Piezo2-expressing HEK293 cell. Top panel, recording at the initial time after establishing the whole-cell mode. Bottom panel, recording 10 min after establish the whole-cell mode. Membrane displacement steps are indicated above Piezo2 MA current traces. B) Summary data of the initial Piezo2 MA currents (n = 6) and the Piezo2 MA currents 10 min after establishing the whole-cell mode (n = 6). *P < 0.05, comparing the Piezo2 MA currents between initial time and 10 min after establishing the whole-cell mode.