Figure 5

Lack of RA current run-up in DRG neurons with internal recording solutions that omitted GTP or replaced GTP with GDP. A) Sample traces of RA currents recorded from a DRG neuron with the internal recording solution that had no GTP. The trace on the left is the initial RA current immediately after establishing the whole-cell mode, and the trace on the right is the RA current recorded 14 min later. Membrane displacement distance was 6 μm. B) Summary data (n = 5) of the RA currents at different time points after establishing the whole-cell mode. C) Sample traces of RA currents recorded with internal solution that contained 0.33 mM GDP but no GTP. Left panel, initial RA current immediately after establishing the whole-cell mode. Right panel, the RA current recorded 12 min later. Membrane displacement distance was 6 μm. D) Summary data (n = 6–9) of the RA currents at different time points after breaking into the whole-cell mode. In both B and D, the slope and the coefficient of determination (r²) of the linear regression are given in the graph. The P value indicates how significant is the slop (i.e. the trend of change) different from zero (i.e. no change).