RA current run-up in DRG neurons with internal recording solution that contained GTPγS. A) Two sample traces show the whole-cell RA currents recorded from a DRG neuron using internal recording solution that contained 0.33 mM GTPγS. Left, the initial RA current immediately after breaking into whole-cell mode. Right, the RA current 24 min later. Membrane displacement distance was 6 μm. B) Time course of RA current run-up with internal recording solution that contained 0.33 mM GTPγS (n = 6–14). The slope and the coefficient of determination (r2) of the linear regression are given in the graph. The P value indicates how significant is the slop (i.e. the trend of change) different from zero (i.e. no change). The number on the top of each symbol indicates the number of cells at each time point. * P < 0.05, ** P < 0.01, comparing the initial RA currents to other time points. C) Comparison of the RA current run-up between recordings with 0.33 mM GTP (n = 20) and with 0.33 GTPγS (n = 6–14). NS, no significant difference, comparing GTP group and GTPγS group. Normalized currents were calculated from B for GTPγS group and from Figure 1D for GTP group.