Change in the dialysate levels of 5-HT induced by electrical stimulation in the mouse DRN at 7 days after sciatic nerve ligation. To investigate the effect of electrical stimulation of the DRN, we evaluated the dialysate 5-HT levels in the PFC in mice by in vivo microdialysis. The mice were subjected to electrical stimulation, sine-wave pulses (100 Hz, 150 μA), for 15 min. The data are expressed as percentages of the corresponding baseline levels ± SEM (n = 4). **p < 0.01 vs. sham. The X-axis shows different time points after stimulation.