Skip to main content

Table 2 Peaks observed in MALDI-TOF MS profile spectra tentatively assigned to known neuropeptides by mass matching

From: A rapid MALDI-TOF mass spectrometry workflow for Drosophila melanogaster differential neuropeptidomics

Obs. m/z(Calc. m/z)

Precursor (UniProtKB Entry name)

Peptide name

Peptide sequence

Previous ref.

915.399 (915.415)

FMRF_DROME

SDNFMRPamide (FMRFamide 9)

R.SDNFMRFa.G

[1, 2, 7, 9, 30]

925.481 (925.490)

ALLS_DROME

Drostatin-3 (Ast-A3)

R.SRPYSFGLa.G

[1, 2, 7, 9, 18]

936.463 (936.473)

TACHY_DROME

TK-3 (dTK-3, APTGFTGMRamide)

R.APTGFTGMRa.G

[1, 2, 18]

942.584 (942.589)

TACHY_DROME

TK-2 (dTK-2, APLAFVGLRamide)

K.APLAFVGLRa.G

[1, 2, 18]

953.529 (953.521)

ALLS_DROME

Drostatin-1 (Ast-A1)

R.VERYAFGLa.G

[9, 18]

961.486 (961.504)

TACHY_DROME

TK-5 (dTK-5, APNGFLGMRamide)

R.APNGFLGMRa.G

[1, 2, 18, 19]

982.622 (982.606)

SNPF_DROME

sNPF-3 (KPQRLRWamide)

R.KPQRLRWa.G

[18]

985.592 (985.588)

SNPF_DROME

sNPF-4 (KPMRLRWamide)

R.KPMRLRWa.G

[18, 19]

996.556* (996.572*)

SNPF_DROME

RLRF peptide 2

R.SPSLRLRFa.G

[1, 2, 9, 18, 19, 30]

1005.557 (1005.512)

FMRF_DROME

SAPQDFVRSamide (FMRFamide 12)

R.SAPQDFVRSa.G

[9, 19, 30]

1015.603 (1015.605)

CP2B_DROME

CAP-2 (CAPA-2)

K.ASGLVAFPRVa.G

[1, 2, 7, 9, 30]

1065.560 (1065.552)

TACHY_DROME

TK-1 (dTK-1, APTSSFIGMRamide)

R.APTSSFIGMRa.G

[1, 2, 9, 18]

1076.560 (1076.568)

TACHY_DROME

TK-4 (dTK-4, APVNSFVGMRamide)

R.APVNSFVGMRa.G

[1, 2, 9, 18, 19]

1112.529 (1112.520)

FMRF_DROME

TPAEDFMRFamide (FMRFamide 7)

R.TPAEDFMRFa.G

[1, 2, 7, 9, 19, 30]

1157.554 (1157.549)

CORZ_DROME

CorazoninAA3-AA11

T.FQYSRGWTNa.G

[1, 2, 30]

1161.551 (1161.558)

AKH_DROME

AKH Peptide (+ C-term GK)

C.pQLTFSPDWGK.R

[9, 19, 30]

1186.538 (1186.511)

DSK_DROME

Drosulfakinin-1 (DSK-1)

R.FDDYGHMRFa.G

[9, 19]

1253.616 (1253.617)

MIP_DROME

Drostatin-B5 (Ast-B4)

R.DQWQKLHGGWa.G

[1, 2, 7, 18, 19]

1276.696 (1276.680)

ALLS_DROME

Drostatin-4 (Ast-A4)

R.TTRPQPFNFGLa.G

[1, 2, 7, 9, 18, 19]

1294.681 (1294.673)

CP2B_DROME

CAP-1 (CAPA-1)

R.GANMGLYAFPRVa.G

[1, 2, 7, 9, 30]

1324.727 (1324.717)

Q59E62_DROME

IFa-2

A.YRKPPFNGSIFa.G

[19]

1329.786 (1329.787)

SNPF_DROME

RLRF peptide 1

K.AQRSPSLRLRFa.G

[9, 18, 30]

1369.644 (1369.629)

CORZ_DROME

Corazonin (Crz Peptide)

G.pQTFQYSRGWTNa.G

[1, 2, 7, 9, 19, 30]

1423.814 (1423.827)

NPLP1_DROME

NAP peptide (+ C-term K) [NPLP1 (APK)]

R.SVAALAAQGLLNAPK.R

[7, 9, 18, 19]

1452.744 * (1452.736*)

CP2B_DROME

CAP-3AA2-AA15

T.GPSASSGLWFGPRLa.G

[1, 2, 9, 19, 30]

1603.841 (1603.835)

MIP_DROME

Drostatin-B3 (Ast-B3)

R.RQAQGWNKFRGAWa.G

[9, 18, 19]

1658.677 (1658.665)

DSK_DROME

Drosulfakinin-2 (DSK-2)

R.GGDDQFDDYGHMRFa.G

[9]

1741.940 (1741.962)

LCK_DROME

Leucokinin (DLK)

R.NSVVLGKKQRFHSWGa.G

[1, 2, 7, 9, 19, 30]

2009.982 * (2009.973*)

PDF_DROME

Neuropeptide PDF

R.NSELINSLLSLPKNMNDAa.G

[19]

2176.201 (2176.188)

CP2B_DROME

CAP Propeptide 3

R.GDAELRKWAHLLALQQVLD.K

[30]

  1. Ion masses observed to be isotopically resolved in both replicate experiments are underlined and bolded. As in Table 1, these reported observed m/z’s are the average of the m/z’s observed between the two replicate MALDI-TOF MS profiling experiments described. Truncated peptides are denoted by superscripts showing amino acids present from the annotated peptide sequence as described in Table 1. Peptide sequences include pre- and post- cleavage residues separated from sequences by a period. *All observed m/z’s listed are predicted to be monoisotopic [M + H]+’s of listed neuropeptides based on the calculated monoisotopic [M + H]+ (shown in parentheses below observed m/z) with the exceptions of m/z of m/z 996.572 and 1452.736 are [M + Na]+’s and m/z 2009.982 is an [M + K]+. C-terminal amidation is denoted by “a”, N-terminal pyroglutamation is denoted by “p”. Abbreviations: Ast – allatostatin, AKH – adipokinetic hormone, CAP - Cardioacceleratory peptide, NPLP1 - Neuropeptide-like precursor 1, PDF - Pigment-dispersing factor, sNPF-Small neuropeptide F, TK - Tachykinin-related peptide.
Back to article page

Molecular Brain

ISSN: 1756-6606