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Table 2 Peaks observed in MALDI-TOF MS profile spectra tentatively assigned to known neuropeptides by mass matching

From: A rapid MALDI-TOF mass spectrometry workflow for Drosophila melanogaster differential neuropeptidomics

Obs. m/z(Calc. m/z) Precursor (UniProtKB Entry name) Peptide name Peptide sequence Previous ref.
915.399 (915.415) FMRF_DROME SDNFMRPamide (FMRFamide 9) R.SDNFMRFa.G [1, 2, 7, 9, 30]
925.481 (925.490) ALLS_DROME Drostatin-3 (Ast-A3) R.SRPYSFGLa.G [1, 2, 7, 9, 18]
936.463 (936.473) TACHY_DROME TK-3 (dTK-3, APTGFTGMRamide) R.APTGFTGMRa.G [1, 2, 18]
942.584 (942.589) TACHY_DROME TK-2 (dTK-2, APLAFVGLRamide) K.APLAFVGLRa.G [1, 2, 18]
953.529 (953.521) ALLS_DROME Drostatin-1 (Ast-A1) R.VERYAFGLa.G [9, 18]
961.486 (961.504) TACHY_DROME TK-5 (dTK-5, APNGFLGMRamide) R.APNGFLGMRa.G [1, 2, 18, 19]
982.622 (982.606) SNPF_DROME sNPF-3 (KPQRLRWamide) R.KPQRLRWa.G [18]
985.592 (985.588) SNPF_DROME sNPF-4 (KPMRLRWamide) R.KPMRLRWa.G [18, 19]
996.556* (996.572*) SNPF_DROME RLRF peptide 2 R.SPSLRLRFa.G [1, 2, 9, 18, 19, 30]
1005.557 (1005.512) FMRF_DROME SAPQDFVRSamide (FMRFamide 12) R.SAPQDFVRSa.G [9, 19, 30]
1015.603 (1015.605) CP2B_DROME CAP-2 (CAPA-2) K.ASGLVAFPRVa.G [1, 2, 7, 9, 30]
1065.560 (1065.552) TACHY_DROME TK-1 (dTK-1, APTSSFIGMRamide) R.APTSSFIGMRa.G [1, 2, 9, 18]
1076.560 (1076.568) TACHY_DROME TK-4 (dTK-4, APVNSFVGMRamide) R.APVNSFVGMRa.G [1, 2, 9, 18, 19]
1112.529 (1112.520) FMRF_DROME TPAEDFMRFamide (FMRFamide 7) R.TPAEDFMRFa.G [1, 2, 7, 9, 19, 30]
1157.554 (1157.549) CORZ_DROME CorazoninAA3-AA11 T.FQYSRGWTNa.G [1, 2, 30]
1161.551 (1161.558) AKH_DROME AKH Peptide (+ C-term GK) C.pQLTFSPDWGK.R [9, 19, 30]
1186.538 (1186.511) DSK_DROME Drosulfakinin-1 (DSK-1) R.FDDYGHMRFa.G [9, 19]
1253.616 (1253.617) MIP_DROME Drostatin-B5 (Ast-B4) R.DQWQKLHGGWa.G [1, 2, 7, 18, 19]
1276.696 (1276.680) ALLS_DROME Drostatin-4 (Ast-A4) R.TTRPQPFNFGLa.G [1, 2, 7, 9, 18, 19]
1294.681 (1294.673) CP2B_DROME CAP-1 (CAPA-1) R.GANMGLYAFPRVa.G [1, 2, 7, 9, 30]
1324.727 (1324.717) Q59E62_DROME IFa-2 A.YRKPPFNGSIFa.G [19]
1329.786 (1329.787) SNPF_DROME RLRF peptide 1 K.AQRSPSLRLRFa.G [9, 18, 30]
1369.644 (1369.629) CORZ_DROME Corazonin (Crz Peptide) G.pQTFQYSRGWTNa.G [1, 2, 7, 9, 19, 30]
1423.814 (1423.827) NPLP1_DROME NAP peptide (+ C-term K) [NPLP1 (APK)] R.SVAALAAQGLLNAPK.R [7, 9, 18, 19]
1452.744 * (1452.736*) CP2B_DROME CAP-3AA2-AA15 T.GPSASSGLWFGPRLa.G [1, 2, 9, 19, 30]
1603.841 (1603.835) MIP_DROME Drostatin-B3 (Ast-B3) R.RQAQGWNKFRGAWa.G [9, 18, 19]
1658.677 (1658.665) DSK_DROME Drosulfakinin-2 (DSK-2) R.GGDDQFDDYGHMRFa.G [9]
1741.940 (1741.962) LCK_DROME Leucokinin (DLK) R.NSVVLGKKQRFHSWGa.G [1, 2, 7, 9, 19, 30]
2009.982 * (2009.973*) PDF_DROME Neuropeptide PDF R.NSELINSLLSLPKNMNDAa.G [19]
2176.201 (2176.188) CP2B_DROME CAP Propeptide 3 R.GDAELRKWAHLLALQQVLD.K [30]
  1. Ion masses observed to be isotopically resolved in both replicate experiments are underlined and bolded. As in Table 1, these reported observed m/z’s are the average of the m/z’s observed between the two replicate MALDI-TOF MS profiling experiments described. Truncated peptides are denoted by superscripts showing amino acids present from the annotated peptide sequence as described in Table 1. Peptide sequences include pre- and post- cleavage residues separated from sequences by a period. *All observed m/z’s listed are predicted to be monoisotopic [M + H]+’s of listed neuropeptides based on the calculated monoisotopic [M + H]+ (shown in parentheses below observed m/z) with the exceptions of m/z of m/z 996.572 and 1452.736 are [M + Na]+’s and m/z 2009.982 is an [M + K]+. C-terminal amidation is denoted by “a”, N-terminal pyroglutamation is denoted by “p”. Abbreviations: Ast – allatostatin, AKH – adipokinetic hormone, CAP - Cardioacceleratory peptide, NPLP1 - Neuropeptide-like precursor 1, PDF - Pigment-dispersing factor, sNPF-Small neuropeptide F, TK - Tachykinin-related peptide.
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