Figure 2

mGluR5a protein expression is increased in cortical astrocytic cultures in response to OGD. A) Representative immunoblot showing increases in mGluR5a protein expression at 0, 2, 4, 6, and 12 h following OGD treatment. B) Quantification of mGluR5a protein expression in primary CD1 mouse cortical astrocytes following 0-12 h OGD. Data is normalized to mGluR5a protein expression at 2 h. Data represents the mean ± SD of 4 independent experiments. C) Real time RT-PCR analysis shows no significant change in mGluR5 mRNA levels in cortical astrocytes after OGD. mGluR5 mRNA levels were normalized to mitochondrial S12 transcript levels and data represents mean ± SD from three independent experiments.