Figure 3

Effect of OGD on InsP formation and ERK1/2 phosphorylation in cortical astrocytes. A) Inositol phosphate (InsP) formation in primary CD1 mouse cortical astrocytes prior to (Ctrl) and following OGD for 4 h in the presence and absence of the mGluR5 inverse agonist MPEP (100 μM). Bonferroni post-hoc tests were performed on three independent experiments (* p<0.05). B) Representative phosph-ERK1/2, total ERK1/2 and actin blots of primary CD1 mouse cortical astrocytes following 0-24 h OGD followed by 24 h reperfusion treated in the absence and presence of the mGluR5 inverse agonist MPEP (100 μM). C) Quantification of ERK1/2 phosphorylation in primary CD1 mouse cortical astrocytes following 0-24 h OGD followed by 24 h re-oxygenation treated in the absence and presence of the mGluR5 inverse agonist MPEP (100 μM). Data was normalized to total ERK1/2 protein expression. Data represents the mean ± SD of 4 independent experiments.