Figure 4

OGD-mediated apoptotic and necrotic cell death in wild type and mGluR5 null cortical astrocytes. A) Photomicrographs of primary cortical astrocytes derived from wild-type C57BL/6 (mGluR+/+) and mGluR5 knockout (mGluR5-/-) littermate embryos that were OGD treated for 0, 12, and 24 hours followed by 24 h re-oxygenation and stained with Hoescht dye to reveal cells dying by apoptosis as evidenced by condensed chromatin/pyknotic nuclei. B) Quantification of apoptotic death of astrocytes derived from mGluR5-/- and mGluR5+/+ embryos in response to 0-24 h OGD followed by 24 h re-oxygenation. Data represent the mean ± SD of 5-8 independent experiments. C) Necrotic cell death measured by LDH assay following 0-24 h OGD followed by 24 h re-oxygenation in cortical astrocytes derived from mGluR5-/- and mGluR5+/+ embryos. Data represent the mean ± SD of 4-7 independent experiments. Data were analyzed by ANOVA followed by Dunnet’s post-hoc testing, * p<0.05 versus untreated control cultures.