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Figure 5 | Molecular Brain

Figure 5

From: Role of metabotropic glutamate receptor 5 signaling and homer in oxygen glucose deprivation-mediated astrocyte apoptosis

Figure 5

Contribution of mGluR5 expression to OGD-induced InsP formation and ERK1/2 phosphorylation. A) Quantification of apoptotic death of astrocytes derived from mGluR5-/- and mGluR5+/+ embryos in absence (DMSO) and presence 3 μM of the selective IP3 receptor inhibitor Xestospongin C (XC) in response to 12 h OGD followed by 24 h re-oxygenation. Bonferroni post-hoc test was performed on three independent experiments,* p<0.05 versus mGluR5+/+ DMSO treated control. B) Representative phosph-ERK1/2, total ERK1/2 and actin blots of primary mouse cortical astrocytes derived from mGluR5+/+ (+) and mGluR5-/- (-) embryos following 0-24 h OGD followed by 24 h re-oxygenation treated. C) Quantification of ERK1/2 phosphorylation in primary mouse cortical astrocytes derived from mGluR5+/+ (+) and mGluR5-/- (-) embryos following 0-24 h OGD followed by 24 h re-oxygenation treated. Data was normalized to total ERK1/2 protein expression. Data represents the mean ± SD of 4 independent experiments.

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