Figure 6

Effect of disrupting mGluR5/Homer interactions on OGD-induced astrocytic cell death. A) Representative immunoblot of Homer1b co-immunoprecipitation with Flag mGluR5a from HEK 293 cells transfected with plasmid cDNAs encoding Homer 1b (1 μg) and Flag-mGluR5a (3 μg) and treated with and without Homer (35 μM) and scrambled (35 μM) Tat-tagged peptides for 1 h. B) Quantification of apoptotic CD1 astrocytic cell death following 12 OGD and 24 h re-oxygenation following the treatment of the astrocytic cultures during the OGD period with Homer and scramble tat-tagged peptides (35 μM). Data represents the mean ± SD of 6 independent experiments. Asterisks represent significant differences by students t test, p<0.05.