Figure 6

Impairment of optic nerve injury-induced gliosis in vps35^+/mmice. (A) Western blot analysis of homogenates from vps35^+/+ and ^+/m optic nerves at indicated ages using indicated antibodies. (B) Illustration of mouse optic nerve injury. P30 vps35^+/+ and ^+/m optic nerves were crushed as indicated. 3-days after injury, optic nerves were sectioned and subjected to immunostaining analysis. (C) Immunostaining analysis of optic nerves using indicated antibodies. Injury sites were marked with yellow arrows. Scale bar, 50 μm. (D) Quantification analysis of data from (C). Immunofluorescence intensity was normalized by the uninjured vps35^+/+ controls. The values of mean +/- SEM (n = 3) were shown. *, P < 0.05, significance difference from the vps35^+/+ control.