DMS stimulates gene expression in hippocampus. (A) Immunohistochemistry for c-fos expression in hippocampal DG region after DMS with P5 treatment for 4 days. (B) Quantitation for c-fos positive cells in per 1 mm3. Values represent mean (±) SEM (n = 4 for each conditions; *: P < 0.05, student’s t-test). (C) mRNA level of fgf1b gene in hippocampus after DMS with P5 for 4 days. Values represent mean (±) SEM (n = 4 for each conditions; *: P < 0.05, student’s t-test). (D) Protein level of Fgf1b after DMS P5 treatment for 4 days. Hippocampal tissue samples were collected from control and animals received P5 daily treatment for 4 days. Samples were homogenized and analyzed by SDS-PAGE using antibodies indicated. (E) Quantification of (D).