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Figure 1 | Molecular Brain

Figure 1

From: Absence of BRINP1 in mice causes increase of hippocampal neurogenesis and behavioral alterations relevant to human psychiatric disorders

Figure 1

Targeted disruption of BRINP1 gene in ES cell and mouse. (A) Construct of the targeting vector for homologous recombination to yield BRINP1-KO mice. The coding region of Brinp1 exon8 (filled box) was disrupted by PGK-neomycin resistance cassette. The probe used for Southern blot analysis is shown together with predicted sizes of hybridizing fragments. Sites of restriction enzymes: Av, AvrII; B, BamHI; H, HindIII; Ps, PstI; RV, EcoRV; Xc, XcmI; Xm, XmaI. (B) Southern blot analysis of BamHI-digested genomic DNA extracted from control (TT2) and positive clone (1–9) of ES cells, and F1 mice produced by crossing chimera mice with C57BL/6J mice. (C) Southern blot analysis of genomic DNA extracted from wild-type (+/+) and BRINP1-KO (−/−) mice. (D) mRNA expression of BRINP family genes in BRINP1-KO mice. Total RNA extracted from adult brain of wild-type (+/+), BRINP1 heterozygous (+/−), and BRINP1-KO (−/−) mice were hybridized with each of Brinp1, Brinp2 and Brinp3 antisense probes. Uniform transfer of RNA was confirmed by methylene blue staining.

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