Figure 1From: Absence of BRINP1 in mice causes increase of hippocampal neurogenesis and behavioral alterations relevant to human psychiatric disordersTargeted disruption of BRINP1 gene in ES cell and mouse. (A) Construct of the targeting vector for homologous recombination to yield BRINP1-KO mice. The coding region of Brinp1 exon8 (filled box) was disrupted by PGK-neomycin resistance cassette. The probe used for Southern blot analysis is shown together with predicted sizes of hybridizing fragments. Sites of restriction enzymes: Av, AvrII; B, BamHI; H, HindIII; Ps, PstI; RV, EcoRV; Xc, XcmI; Xm, XmaI. (B) Southern blot analysis of BamHI-digested genomic DNA extracted from control (TT2) and positive clone (1–9) of ES cells, and F1 mice produced by crossing chimera mice with C57BL/6J mice. (C) Southern blot analysis of genomic DNA extracted from wild-type (+/+) and BRINP1-KO (−/−) mice. (D) mRNA expression of BRINP family genes in BRINP1-KO mice. Total RNA extracted from adult brain of wild-type (+/+), BRINP1 heterozygous (+/−), and BRINP1-KO (−/−) mice were hybridized with each of Brinp1, Brinp2 and Brinp3 antisense probes. Uniform transfer of RNA was confirmed by methylene blue staining.Back to article page