Figure 3

Histological analyses of the treatment effects on CST regeneration in the injured spinal cord using BDA staining. J shows a representative axial section of the spinal cord at the C4 level (rostral to the lesion site), and K shows a representative image of a section at the L4 level (caudal to the lesion site) (Scale bars = 500 μm). Based on these sections, we confirmed that the labeling efficiency of BDA tracing in each animal was sufficient. A, B and C show representative sagittal sections of the BDA staining in each group. D/E show magnified images of the boxed area in A. Similarly, F/G show magnifications of the areas in B, and the images in H/I are magnifications of the areas in C. Scale bars = 1,000 μm in A-C and 100 μm in D-I. No obvious CST regeneration beyond the lesion epicenter was observed in any of the groups examined (L). Statistical analyses were performed using one-way ANOVA and Bonferroni post hoc analyses. Data are presented as the mean ± S.E.M. The images in M are representative of other sagittal sections from each group. Scale bars = 1,000 μm in the images on left side of M and 100 μm in the magnified images of M.