Expression profile and glycosylation pattern of human acetylcholinesterase (hAChE) in human embryonic kidney 293 (HEK293) cells. Expression profiles of read-through AChE (AChE-R) and synaptic AChE (AChE-S) in the cell lysate (A) and culture medium (B) of HEK293 cells transfected with hAChE-R or with hAChE-S. The AChE-S proteins display a band with molecular weight of approximately132 kDa, and another two bands with molecular weight of about 68 and 66 kDa, respectively, whereas the AChE-R proteins display two bands with molecular weight of about 70 and 68 kDa, respectively. A’ illustrates the two lower molecular weight bands of AChE-S and AChE-R. C. Immunoblots of AChE-R generated by processing the cell lysates with various N- and O-glycohydrolases (N = peptide N-glycosidase F, an N-linked glycohydrolase; O = O-glycosidase and S = sialidase, two O-linked glycohydrolases). D. Immunoblots of AChE-S generated by processing lysates of transfected HEK293 cells without additional treatment (vehicle control [ctrl]) or with tunicamycin treatment (Tunic.).