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Figure 6 | Molecular Brain

Figure 6

From: Interaction of Acetylcholinesterase with Neurexin-1β regulates Glutamatergic Synaptic stability in Hippocampal neurons

Figure 6

Increase in extracellular acetylcholinesterase (AChE) decreases glutamate-induced currents in primary neurons. A. Representative images of cultured hippocampal neurons transfected with complementary DNA encoding AChE. Left panel shows cultured neurons under a light microscope, and right panel displays a GFP fluorescent image of the same field. Red arrow indicates the cell body of a transfected cell. B. Traces of glutamate-induced current in a control (Vector) neuron, a neuron expressing read-through AChE (AChE-R) and a neuron expressing synaptic AChE (AChE-S). C. Amplitude of glutamate-induced current in control neurons (expressing GFP only), AChE-R-expressing and AChE-S-expressing neurons (control: 1013 ± 181 pA, n = 11 neurons; AChE-R: 532 ± 69 pA, n = 11 neurons; AChE-S: 580 ± 72 pA, n = 12 neurons; * P < 0.05). D. Amplitude of glutamate-induced current in control neurons and in neurons surrounding the AChE-R- and AChE-S-expressing neurons (control: 1881 ± 146 pA, n = 7 neurons; surrounding AChE-R-expressing neurons: 1157 ± 116 pA, n = 7 neurons; surrounding AChE-S-expressing neurons: 1236 ± 69 pA, n = 7 neurons; * P < 0.05). E. Amplitude of glutamate-induced current in control neurons and in neurons treated with 3.0 units/ml human AChE-S (hAChE 3), 1.0 unit/ml human AChE-S (hAChE 1) or 10 μM physostigmine (Physo) (control: 645 ± 68 pA, n = 11 neurons; hAChE 3: 413 ± 45 pA, n = 11 neurons; hAChE 1: 367 ± 90 pA, n = 9 neurons; Physo.: 587 ± 62 pA, n = 10 neurons; * P < 0.05). F. Amplitude of glutamate-induced current in control neurons and in neurons treated with conditioned medium containing catalytically inactive AChE-S (AChE-Sin) (control: 2.6 ± 0.29 nA, n = 8 neurons; AChE-Sin: 1.3 ± 0.22 nA, n = 11 neurons; * P < 0.05).

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