Figure 1
From: Zinc transporter-1 concentrates at the postsynaptic density of hippocampal synapses
Synaptic targeting of ZnT1. (A) Bright field immunostaining of ZnT1 in mouse CA1 region. Neuronal perikarya and apical dendrites (arrowheads) were labeled. s.o. stratum oriens; s.p. stratum piramidale; s.r. stratum radiatum. Scale bar, 100 μm. (B) Confocal images of double stained dendrites of cultured pyramidal neurons. Scale bar, 5 μm. (C) Left, western blot for ZnT1 in nuclear (N) and cytoplasmic (C) extracts (20 μg per lane) from adult hippocampus. MW was assessed with biotinylated protein ladder. Right, Synaptic and extrasynaptic fractions (10 μg per lane) were probed for ZnT1, stripped, and reprobed for actin and PSD-95.