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Figure 2 | Molecular Brain

Figure 2

From: Optimization of AAV expression cassettes to improve packaging capacity and transgene expression in neurons

Figure 2

Comparison of transgene expression with modification of polyadenylation signal. A. Schematic drawings of EGFP expression cassettes having modifications in polyadenylation signal. (L: SV40 late polyadenylation signal; SL: SV40 late polyadenylation signal upstream element + SV40 late polyadenylation signal; A: synthetic polyadenylation signal, SA: SV40 late polyadenylation signal upstream element + synthetic polyadenylation signal; SSA: double copy of SV40 late polyadenylation signal upstream element + synthetic polyadenylation signal). B-C. Representative images of fluorescence (B) and western blot analysis (C) of EGFP and tdTomato expression. Scale bar, 200 μm. D. Summary graph shows that CW3SL containing WPRE3, an additional SV40 late polyadenylation signal upstream element followed by SV40 late polyadenylation signal, drives highest EGFP expression level. CW3B and CW3L drive significantly higher expression than CW3A (P < 0.01), while CW3SL drives significantly higher expression than CW3A (P < 0.001), CW3SA (P < 0.01), and CW3SSA (P < 0.05) (*P < 0.05, **P < 0.01, ***P < 0.001, ANOVA Bonferroni’s post-hoc comparison).

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