Utilization of CW3SL expression cassette for large transgene expression. A. Western blot analysis of p110γ-EGFP fusion protein expression by CWB-p110γ-EGFP and CW3SL-p110γ-EGFP. B. Quantification of the western blot analysis. p110γ-EGFP expression level was normalized to that of tubulin, which was then normalized to the average expression level by CWB-p110γ-EGFP. C. The percentage of transcripts in mouse hippocampus with coding sequence length within every 0.4 kb. Transcripts expressed in mouse hippocampus were selected based on RNA sequencing data (analyzed based on Ensembl Genes version 67, NCBIM37). The transcripts in the range of 2.4-4 kb (striped bars) and those in the range of 3.6-4 kb (darker and striped bar) that exceed the packaging capacity of conventional AAV expression cassettes and CWB cassette, respectively, become available using CW3SL cassette.