Figure 2
From: Disruption of the nuclear p53-GAPDH complex protects against ischemia-induced neuronal damage
The nuclear translocation of GAPDH upon GluR2 AMPA receptor activation depends on Siah-1. A. Western blots verification of Siah 1 siRNA knockdown of the Siah-1 expression in HEK 293 T cells. B. Western blots analysis of GAPDH expression in the nucleus with/without glutamate treatment in the presence/absence of the Siah1 siRNA (Santa Cruz) in HEK-293 T cells expressing GluR1/2 subunits. LaminB1 was used as the nuclear marker. C &D, Quantification of nuclear expression of GAPDH with/without glutamate treatment in the presence/absence of the Siah1 siRNA (Santa Cruz), three Siah1 siRNA (#1, #2, #3, OriGene) and scrambled negative control siRNA (scramble, OriGene). *p < 0.05; significantly different from control group (n = 3 per group). #p < 0.05; significantly different from glutamate group (n = 3 per group). ANOVA, followed by post-hoc SNK test.