Figure 3
From: Disruption of the nuclear p53-GAPDH complex protects against ischemia-induced neuronal damage
Identification of GAPDH-p53 interaction fragment of GAPDH molecule. A, CoIP assay of GAPDH/p53 using solubilized nuclear proteins extracted from rat hippocampal slices with/without glutamate treatment. B, Schematic representation of GST-fusion proteins encoding truncated GAPDH segments (gray colored bars indicate the positive fragments). C, Western blots of cortical nuclear p53 after affinity precipitation by different GST-GAPDH fragments (GST and GST-GAPDH, GST-GAPDH1 and GST-GAPDH2, GST- GAPDH2–1 and GST-GAPDH2–2, GAPDH2–2–1 and GAPDH2–2–2, GAPDH2–2–1–1, GAPDH2–2–1–2 and GAPDH2–2–1–3) D, CoIP of nuclear p53 by GAPDH primary antibody in HEK-293 T cells co-expressing GluR1/2 subunits with GAPDH2–2–1–1 peptide.