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Figure 2 | Molecular Brain

Figure 2

From: Extracellular signal-regulated kinase (ERK) activation is required for itch sensation in the spinal cord

Figure 2

Expression of pERK and c-Fos in the spinal dorsal horn 30 min after injection of histamine, compound 48/80, chloroquine or SLIGRL-NH2. (A-D) pERK is expressed in the lateral dorsal horn of mice injected with histamine and compound 48/80 (A, C) but not with chloroquine and SLIGRL-NH2 (B, D) in the nape. (E, F) Similar c-Fos expression is observed in the dorsal horn after injection of chloroquine or histamine in the nape. (G) Comparison of the number of pERK-positive cells in the dorsal horn of mice treated with PBS, histamine and chloroquine. One way ANOVA; P < 0.001; **P < 0.01; n = 5 for each group. (H) Comparison of the number of c-Fos-positive cells in the dorsal horn of mice treated with chloroquine or histamine as shown in (E, F). Student’s t-test; P > 0.05; n = 5 for each group. (I) pERK is expressed in the medial dorsal horn of mice that received histamine injection in a hind paw. (J) pERK-positive cells are distributed in the dorsal part of the medullary dorsal horn of mice with histamine injection in the cheek region. DH, dorsal horn; df, dorsal fasciculus; lf, lateral fasciculus; MDH, medullary dorsal horn; t, spinal trigeminal tract. Scale bars, 100 μm in F (applies to A-F and I) and 200 μm in J.

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