Figure 5

Specific expression of pERK in the superficial spinal neurons in histamine-injected and repeated DNFB-painted mice. (A, B) The vast majority of pERK-positive cells are located dorsally to the PKCγ-positive cells in histamine-inject mice. (C-E) pERK expression co-localizes with NeuN (arrowheads in C), but not with GFAP (D) or OX42 (E) in mice injected with histamine. (F-H) Laser confocal images show co-localization of NeuN and pERK (arrowheads in F), but no co-localization of pERK with GFAP (G) or Ox42 (H) in the spinal dorsal horn of repeated DNFB-painted mice. I, lamina I; IIi, inner lamina II; IIo, outer lamina II; III, lamina III. Scale bars, 100 μm (A, B,) and 50 μm (C-H).