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Figure 8 | Molecular Brain

Figure 8

From: Extracellular signal-regulated kinase (ERK) activation is required for itch sensation in the spinal cord

Figure 8

Histamine- and DNFB-, but not chloroquine-induced high excitability of spinal dorsal horn neurons is suppressed by U0126. Spinal slices were prepared 30 min after injection of PBS (20 μl), histamine (100 μg in 20 μl PBS), or chloroquine (80 μg in 20 μl PBS) into hind paw, or the last painting of DNFB on hind paw. (A, B) Voltage-clamp recording from outer laminar II neurons shows that the frequency but not the amplitude of sEPSC is increased in mice treated with histamine, chloroquine or DNFB. Six mice were used in each set of experiment, and recorded neuron numbers are shown in (B). One way ANOVA, P < 0.01; **P < 0.01. (C, D) Current-clamp recording shows the decreased threshold of action potential (C) and increased frequency of action potential triggered by current injection (D) in histamine-, chloroquine- and DNFB-treated mice. Bath application of U0126 (1 μM) only prevents the changes in histamine- and DNFB-treated mice but not those treated with chloroquine. Five mice were used in each experiment and recorded neuron numbers are shown in (C, D). Student’s t-test, *P < 0.05. (E) Samples of action potential frequency changes after bath application of U0126 in control mice and histamine-injected mice. AP, action potential.

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