Alterations in blood–spinal cord barrier (BSCB) integrity after spinal cord ischemia reperfusion I/R injury. (A) Effects of spinal cord I/R injury on BSCB permeability measured by Evans blue (EB) extravasation. Almost no red fluorescence was seen in spinal cord of Sham group at 12 and 36 h after injury. Much more red fluorescence, especially in the gray matter could be seen in I/R group at 12 h after injury, which was even stronger at 36 h postoperatively. And EB red extravasation was significantly weakened in the groups pretreated with minocycline, TAK-242 and PDTC, whereas EB extravasation was worsen by intrathecal injection with LPS at above time points. (B) Percentage of EB extravasated area. (C) EB content of the spinal cord (μg/g). (D) Quantification of the water content of the spinal cord (edema). All data are presented as mean ± SEM (n = 8 per group). Scale bars are 200 μm. **P < .01 compared to Sham group; ##P < .05 compared to I/R group.