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Figure 1 | Molecular Brain

Figure 1

From: MAP1B rescues LRRK2 mutant-mediated cytotoxicity

Figure 1

The interaction and phosphorylation of LRRK2 and LC1. (A) Cellular co-localisation of endogenous LRRK2 and MAP1B (LC1). SKNSH cells were labelled with LRRK2 (green) and LC1 (red) antibodies and signals were detected by immunofluorescence. Detailed figure in Additional file 1. (B) LRRK2 kinase assay. The LRRK2 wild-typed (WT) kinase interacted and phosphorylated the LC1 domain unlike the LRRK2 kinase-dead protein. (C) Western blotting of LRRK2 and LC1 proteins after a kinase assay. Both LRRK2 and LC1 proteins were probed for phospho-threonine and phospho-serine signals respectively.

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Molecular Brain

ISSN: 1756-6606