Generation and validation of mGluR3 KO mice. (a) WT and KO alleles: Closed boxes indicate the approximate positions of the 5′ and 3′ probes. The closed triangle indicates the loxP sequence. (b) Southern blots of Bam H I and Sph I-digested genomic DNA were hybridized with the 5′ probe and 3′ probe shown in (a), respectively. The DNA bands indicated by the arrows correspond to the Bam H I and Sph I-fragments. (c) RT-PCR analysis of mGluR3 expression in the brains of C57BL/6J (B6) and KO mice using specific primers located in exons 3 and 4 of Grm3. Total RNAs were isolated from the brains of B6 and KO mice. A PCR product of 695 bp was detected in B6, but not in KO mice.