Both chronic immobilization and unpredictable stress cause long lasting loss of tonic but not phasic inhibition in lateral amygdala. A, Representative traces showing the tonic GABAAR currents in the projection neurons (PNs) of lateral amygdala (LA) from control mice (upper) and mice experiencing 10 days of recovery from chronic immobilization (CIS, middle) or unpredictable stress (CUS, bottom). The tonic currents were measured as the amplitude difference between the dashed lines indicating the average holding current before and after BIC application. B, Summary of the tonic GABAAR currents in control, CIS and CUS mice. C, Representative traces showing the spontaneous inhibitory postsynaptic currents (sIPSCs) in LA PNs from control (upper), CIS (middle) and CUS (bottom) mice. D-G, Summary of the sIPSCs amplitude (D), frequency (E), 10-90% rise time (F) and decay constant (G) in LA PNs from control, CIS and CUS mice. ** p < 0.01 (unpaired t test).