The alteration of tonic GABA
R currents by chronic immobilization stress (CIS) varies with the daily immobilization duration and the total days of immobilization exposure. A, Representative traces showing the tonic GABAAR currents in CIS mice subjected to 15 minutes (upper) or 2 hours (bottom) of daily immobilization. B, Comparisons of the tonic GABAAR currents between control mice and CIS mice subjected to daily immobilization with different durations. C, Procedures used to treat the mice with different days of immobilization stress (IS) followed by 10 consecutive days of stress-free recovery. The tonic GABAAR currents in the LA PNs were recorded upon the cessation of recovery. D, Representative traces showing the tonic GABAAR currents in different groups of IS mice. E, Comparison of the tonic GABAAR currents between control mice and different groups of IS mice. *p < 0.05; **p < 0.01.