Table 2 Primers used to create single nucleotide variation in nAChR α6 subunit cDNA
Sl. no | Primer | Oligonucleotide sequence (5’ → 3’) |
|---|---|---|
1. | hα6(S43P) | ctcttccacaaactgtttCctcattacaaccagttc |
2. | hα6(N46K) | ctgttttctcattacaaGcagttcatcaggcctg |
3. | hα6(D57N) | gtggaaaacgtttccAaccctgtcacggtac |
4. | hα6(R87C) | gaaaccaatttgtggctgTgtcacatctggaatgatt |
5. | hα6(D92E) | cgtcacatctggaatgaGtataaattgcgctggg |
6. | hα6(R96H) | ctggaatgattataaattgcActgggatccaatggaatatg |
7. | hα6(E101K) | gcgctgggatccaatgAaatatgatggcattgag |
8. | hα6(A112V) | gactcttcgcgttcctgTagataagatttggaagc |
9. | hα6(S156R) | ccagctatttttaagagGtcctgccctatggatatc |
10. | hα6(N171K) | ctttttcccttttgatcatcaaaaGtgttccctaaaatttggttcctgg |
11. | hα6(A184D) | cctggacgtatgacaaagAtgaaattgatcttctaatc |
12. | hα6(D199Y) | ggatcaaaagtggatatgaatTatttttgggaaaacagtgaatg |
13. | hα6(N203T) | gatatgaatgatttttgggaaaCcagtgaatgggaaatcattgatg |
14. | hα6(I226T) | caaatacaactgttgtgaagagaCatacacagatataacctattc |
15. | hα6(S233C) | gatatacacagatataacctattGtttctacattagaagattgccg |