Table 3 A, Summary of biophysical parameters of hCav3.1 and hCav3.1 domain IV mutants and B, Summary of biophysical parameters of hCav3.1 domain II mutants in the absence or presence of 1 μM ProTx I
From: Block of T-type calcium channels by protoxins I and II
A | |||||
|---|---|---|---|---|---|
DOM IV mutant | IC50 Tonic ProTx I (μM) | V0.5act (mV) Con | V0.5act (mV) ProTx I (1μM) | Vh(mV) Con | Vh (mV) ProTx I (1 μM) |
hCav3.1 wt | 0.64 | -52.3 | -52.3 | -74 | -76 |
hCav3.1 V1618A | 0.66 | -51.5 | -46.8 | -79* | -77 |
hCav3.1 Q1631K | 0.92 | -53.2 | -54.3 | -84* | -82 |
hCav3.1 S1659A | 1.29 | -53.1 | -50.8 | -77 | -79 |
hCav3.1 M1696V | 1.27 | -51.5 | -49.1 | -74 | -79 |
B | |||||
DOM II mutant | Current block (%) ProTx l (1 μM) | V 0.5 act (mV) Con | V 0.5 act (mV) ProTx I (1 μM) | Vh(mV) Con | Vh (mV) ProTx l (1 μM) |
Q826A | 40 | -53.6 | -51.2 | -75.2 | -73.6 |
G827D | 38 | -52.4 | -52.7 | -77 | -72 |
QG826-7 AD | 40 | -51 | -48.2 | -74 | -76.3 |