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Table 3 A, Summary of biophysical parameters of hCav3.1 and hCav3.1 domain IV mutants and B, Summary of biophysical parameters of hCav3.1 domain II mutants in the absence or presence of 1 μM ProTx I

From: Block of T-type calcium channels by protoxins I and II

A
DOM IV mutant IC50 Tonic ProTx I (μM) V0.5act (mV) Con V0.5act (mV) ProTx I (1μM) Vh(mV) Con Vh (mV) ProTx I (1 μM)
hCav3.1 wt 0.64 -52.3 -52.3 -74 -76
hCav3.1 V1618A 0.66 -51.5 -46.8 -79* -77
hCav3.1 Q1631K 0.92 -53.2 -54.3 -84* -82
hCav3.1 S1659A 1.29 -53.1 -50.8 -77 -79
hCav3.1 M1696V 1.27 -51.5 -49.1 -74 -79
B
DOM II mutant Current block (%) ProTx l (1 μM) V 0.5 act (mV) Con V 0.5 act (mV) ProTx I (1 μM) Vh(mV) Con Vh (mV) ProTx l (1 μM)
Q826A 40 -53.6 -51.2 -75.2 -73.6
G827D 38 -52.4 -52.7 -77 -72
QG826-7 AD 40 -51 -48.2 -74 -76.3
  1. Asterisks denote statistical significance relative to wild type hCav3.1 [*p < 0.05].